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The M1 (CD86 + ) to M2 (CD206 + ) ratio of hPBMC-macrophages was analyzed by flow cytometry using CD45-APC/CY 7 (2D1, 368516), CD68-BV421 (Y1/82 A, 333827), CD86-PE (BU63, 374206), and CD206-APC (15-2, 321110) antibodies (BioLegend, USA)